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Structured Review

The Nest Group macrospin c18 column
Macrospin C18 Column, supplied by The Nest Group, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/macrospin+c18+column/c18+macrospin+columns/pm40618317-80-13-16
Average 90 stars, based on 1 article reviews
macrospin c18 column - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Phospho-proteomics:

Article Title: Proteomic study of the membrane components of signalling cascades of Botrytis cinerea controlled by phosphorylation
Article Snippet: .. Crude phosphopeptide mixtures were desalted using a MacroSpin C18 column (The Nest Group, Inc., Southborough, MA). .. 45% of each enriched phosphopeptide mixture was analysed using a LTQOrbitrap Velos Pro mass spectrometer (Thermo Fisher Scientific, San Jose, CA) coupled to a nano-LC (Proxeon, Odense, Denmark) equipped with a reversed-phase chromatography 25 cm column with an inner diameter of 75 μm, packed with 1.9 μm C18 particles (Nikkyo Technos, Japan).

other:

Article Title: Protein cycling in the eastern tropical North Pacific oxygen‐deficient zone: A de novo‐discovery peptidomic approach
Article Snippet: Peptides are identified using a de novo-discovery approach in suspended and sinking particles from the eastern tropical North Pacific oxygen-deficient zone (ODZ) and in a culture of a dominant autotroph from the region, the cyanobacterium Prochlorococcus.. The benchmarking experiment with Prochlorococcus shows de novo peptides to be taxonomically specific, and thus of value in augmenting database-driven approaches.. Analysis of the suspended and sinking particles using the de novo-discovery approach reveals the presence of fungal proteins in deep sinking particles that were not in our original search database, contributing to growing recognition that fungi may play important roles in marine organic matter cycling.

Chromatography:

Article Title: The cochaperone CHIP marks Hsp70- and Hsp90-bound substrates for degradation through a very flexible mechanism
Article Snippet: The resulting protein extract was diluted 6-fold with 200 mM NH 4 HCO 3 and digested with 20 μg trypsin (V5113; Promega; overnight, 37 °C). .. The peptide mix was acidified with formic acid and desalted in a MacroSpin C18 column (The Nest Group), and 97.5% of the sample was further fractionated by strong cation exchange chromatography (SCX; Empore cation-SR, 3 M) in a procedure adapted from Rappsiber and collaborators . ..



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Image Search Results


Journal: iScience

Article Title: Pancreatic cancer organoid-screening captures personalized sensitivity and chemoresistance suppression upon cytochrome P450 3A5-targeted inhibition

doi: 10.1016/j.isci.2024.110289

Figure Lengend Snippet:

Article Snippet: MacroSpin column PROTO 300 C18 , Harvard Apparatus , Cat# 74-4107.

Techniques: Recombinant, Membrane, Saline, Electron Microscopy, Staining, Lysis, Extraction, Protease Inhibitor, Sequencing, Modification, Bicinchoninic Acid Protein Assay, Software, High Content Screening